Cytochrome b/b6 non-covalently binds two heme groups, known as b562 and b566. One of them is gene for cytochrome b (CytB). A total of 100 tortoise shell specimens collected from the National Council of Agriculture (COA), Taiwan, were used in this study. Using these primers, 426 bp fragments of a mitochondrial(mt) cytochrome b gene were amplified by polymerase chain reaction (PCR), directly sequenced, and compared among Aspergillus fumigatus, A. flavus, A. niger, A. terreus and Emericella … Further, the CYBB (cytochrome b‐254 beta‐chain) gene (OMIM #300481) is situated on the short arm of the X chromosome at position 21.1, containing 13 exons and spanning 30 kb. These primers were mixed in the ratio of 1: 0.2: 3:3: 0.6: 3: 0.6: 2 for SIM: G: Ch: C: B: S: P: H and used together for the multiplex PCR. Three unknown samples of animal remains were identified by this system. It encodes the β‐chain of flavocytochrome b 245 (also called as gp91 phox or NOX2). Skuza L, Filip E, Szućko I, Bocianowski J. Int J Mol Sci. NIH The most of animal mtDNA is coding 37 genes [1]. Species identification was carried out by nucleotide sequence analysis of the cytochrome b (cytb) gene. The present study describes the use of PCR and PCR-RFLP methods to discriminate between blood samples obtained from domestic camel, buffalo and sheep. Species identification by cytochrome b gene: Casework samples NLM This method allowed the identification of fish species in all samples. To study the identification and phylogeny of pathogenic isolates of Aspergillus, we designed primers from known cytochrome b aminoacid sequences. Species identification using the cytochrome b gene of commercial turtle shells. The evolution dynamics of this gene and the biochemistry of its protein product are also Identification of endangered petrel species from poor quality feather debris using cytochrome b sequences. Forensic Sci Int 2003; 136: 1-11. 2018 Aug 17;3(2):925-932. doi: 10.1080/23802359.2018.1501284. The conservation of animals species included in this study were: the formosan gem-faced civets, leopard cats, tigers, clouded leopards, lion, formosan muntjacs, formosan sika deers, formosan sambars, formosan serows, water buffalo, formosan pangolins and formosan … The identification of the presence of C. paca in the samples was 100% reliable. Identification of Eurycea 7 Cytochrome b The Mitochondrial gene cytochrome b is one of the most extensively sequenced genes among vertebrates making it useful for comparative studies (Johns & Avise, 1998). The applicability of this method to the forensic field is demonstrated by simulated casework conditions where different types of samples including problematic specimens such as hair, bone samples, bristles and feathers were investigated to identify the species. NCI CPTC Antibody Characterization Program. DNA barcoding as a tool for robust identification of cervids of India and its utility in wildlife forensics. 2020 Dec 10;21(24):9421. doi: 10.3390/ijms21249421. The sequences were analyzed using the Sequence Analysis/Seqscape v2.1 software (Applied Biosystems). and Tsai, L.C. | 8. This DNA sequence may not only assist in the identification of the unknown sample, but can be used to determine the phylogenetic relationships of rhinoceros species. Rajpoot A, Kumar VP, Bahuguna A, Singh T, Joshi S, Kumar D. Mitochondrial DNA B Resour. The conservation of animals species included in this study were: the formosan gem-faced civets, leopard cats, tigers, clouded leopards, lion, formosan muntjacs, formosan sika deers, formosan sambars, formosan serows, water buffalo, formosan pangolins and formosan macaques. Mol Biol Evol. Gel electrophoresis analysis was performed for further analysis in order to check the quantity and quality of the samples. and Linacre, A. and Hsieh, H.M. ( 2009 ) Species identification using the cytochrome b gene of commercial turtle shells. 2020 Jan 7;5(1):388-389. doi: 10.1080/23802359.2019.1704184. Mitochondrial DNA B Resour. USA.gov. Sequences were aligned by Pileup program of GCG computer package, and the phylogenetic tree was constructed by the neighbor-joining method. Forensic Science International, 122 (1). mammals, birds, reptiles, amphibians and fishes). Cytochrome b/b6 is an integral membrane protein of approximately 400 amino acid residues that probably has 8 transmembrane segments. A DNA technique has been established for the identification to species level of tortoises. Hsieh HM, Tsai CC, Chiang HL,Tsai LC, Huang NE, Shih RTP, Linacre A, Lee JCI. hopeful technique for meat species identification. mammals, birds, reptiles, amphibians and fishes). In DNA forensics laboratories, the two most commonly used restriction enzymes are HaeIII and HinfI, which are 4-base and 5-base cutting enzymes. The present study was conducted to identify cattle (Bos taurus) and buffalo (Bubalus bubalis) by using mitochondrial cytochrome-b (Cyt-b) gene.Size of the gene is 1140 bp, but we amplified 359 bp that is cleaved by specific … Sequence analysis of PCR products from a conserved region of the cytochrome b gene was used to identity fish species belonging to the families Gadidae and Merluccidae in 18 different processed fish products. DNA was prepared for sequencing by PCR. Cytochrome b gene for species identification of the conservation animals Hsieh, H. and Chiang, H. and Tsai, L. and Lai, S. and Huang, N. and Linacre, A.M.T. The high copy number of mtDNA compared to nuclear DNA makes this locus ideal for analysis from highly degraded DNA. A comparative summary of genetic distances in the vertebrates from the mitochondrial cytochrome b gene. Species Identification of Kachuga tecta Using the Cytochrome b Gene. The results of sequence comparison showed that the percentage range of sequence diversity in the same species was from 0.25 to 2.74%, and that between the different species was from 5.97 to 34.83%. By continuing you agree to the use of cookies. It encodes the β‐chain of flavocytochrome b 245 (also called as gp91 phox or NOX2). Validation of cytochrome b sequence analysis as a method of species identification. Lima NGDS, do Carmo AO, de Souza RCC, Kalapothakis E, Eterovick PC. 3. 2016 Jul;23:159-165. doi: 10.1016/j.fsigen.2016.05.001. The partial sequence of cytochrome b gene adopted in this study proved to be usable for animal identification. One of them is gene for cytochrome b (CytB). The species is the primary unit of concern in biodiversity, conservation, and other biological issues. As an alternative to direct DNA sequencing of PCR products, random PCR-RFLP is an efficient technique to discriminate between species. The gene is 1,140 basepairs long in most vertebrates and shows little intraspecies variation, but sufficient interspecies variation. The most of animal mtDNA is coding 37 genes [1]. A novel strategy for avian species identification by cytochrome b gene. Mitochondrial DNA (mtDNA), especially the gene for cytochrome b (MT-CYB), has been found to be highly informative for species identification. Consequently, it was found that the PCR‐RFLP method was sufficient for rapidly screening cod products, and in cases that require conclusive identification, phylogenetic … Hsieh HM, Huang LH, Tsai LC, Liu CL, Kuo YC, Hsiao CT, Linacre A, Lee JCI. Cyt b gene is a gene that is often used to compare multiple phylogenetic species in the same genus or family, the diversity of the cyt b gene has been used to detect the source of milk derived from cattle (Bos), | Nakaki S, Hino D, Miyoshi M, Nakayama H, Moriyoshi H, Morikawa T, Itohara K (2007) Study of animal species (human, dog and cat) identification using a multiplex single-base primer extension reaction in the cytochrome b gene. This method allowed the identification of fish species in all samples. Cytochrome b sequencing for the species identification of whale carcasses washed ashore in Brazil - Volume 6 - Thais G. Corrêa Sholl, Jailson Fulgencio de Moura, Paulo Henrique Ott, Cibele R. Bonvicino, Estéfane C. Reis, Davi Castro Tavares, Salvatore Siciliano Levels of sequence variation in mitochondrial cytochrome-b gene were examined to ascertain if this molecule can provide a reference point in making decisions concerning species-level distinctions.DNA-sequence data from 4 genera of rodents (Neotoma, Reithrodontomys, Peromyscus, and Sigmodon) and 7 genera of bats (Artibeus, Carollia, Chiroderma, Dermanura, … A partial DNA sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to Taiwan. The aim of the study was to identify biological specimens from diverse vertebrate animals by extracting and amplifying DNA from 44 different animal species covering the 5 major vertebrate groups (i.e. Sequence analysis of PCR products from a conserved region of the cytochrome b gene was used to identity fish species belonging to the families Gadidae and Merluccidae in 18 different processed fish products. Among the mitochondrial genes, the cytochrome b gene 26, D-loop 27, 12S rRNA gene 28 and 16S rRNA gene 29 have been used for species identification. Epub 2007 Feb 21. Species identification by cytochrome b gene: Casework samples Abstract. Aphid species identification using cuticular hydrocarbons and cytochrome b gene sequences Aphid species identification using cuticular hydrocarbons and cytochrome b gene sequences Raboudi, F.; Mezghani, M.; Makni, H.; Marrakchi, M.; Rouault, J. D.; Makni, M. 2005-03-01 00:00:00 Abstract: In Tunisia, four major aphid species have been identified based on adult … J Forensic Sci 2006; 51: 52-6. The aim of the study was to identify biological specimens from diverse vertebrate animals by extracting and amplifying DNA from 44 different animal species covering the 5 major vertebrate groups (i.e. Parasitol Res. Forensic Sci Int Genet. pp. Traditionally the cytochrome b (cyt b) gene was used for species identification, but in 2003 the cytochrome c oxidase subunit 1 (CO1) gene was introduced … HHS Because adulteration of the drug with substances from other animals such as horses, cattle, and pigs has been found, we examined PCR methods based on the sequence of the cytochrome b gene for source species identification. Water Res. Pinheiro a,b b a Instituto Nacional de Medicina Legal, Delegac¸a˜o do Porto, Portugal Faculdade de Cieˆncias da Sau´de, Universidade Fernando Pessoa, Portugal Abstract. Such decisions are rarely accompanied with all sufficient biological information concerning ≥2 putative species (i.e., sympa… We developed a species identification based on sequencing of the cytochrome b (cyt b) gene directly from the DNA extracted from the clinical specimen. It is considered to be most useful in determining relationships within families and genera. Additionally, we conducted a more strict check by phylogenetic analysis on imported cod fish products with 13 standards (including 4 additional cod species from GenBank) using the mitochondrial cytochrome b gene (385 nt). 2. SPInDel Analysis of the Non-Coding Regions of cpDNA as a More Useful Tool for the Identification of Rye (Poaceae: A novel droplet digital PCR human mtDNA assay for fecal source tracking. The development of the Polymerase Chain Reaction and primers are an integral part of the modern DNA sequencing process. The sequences derived were used to identify the … The unambiguous identification of rhino products using a 402 bp fragment of cytochrome b gene was investigated. In the initial identification of species from unknown powdered material, all the … which is in the field of taxonomy and the identification of species. Gonçalves R, Parik J, Freitas AI, Jesus J, Villems R, Brehm A. Forensic Sci Int. Primer pairs were designed to amplify partial DNA fragments of cytochrome b … The sequences were analyzed using the Sequence Analysis/Seqscape v2.1 software (Applied Biosystems). We further sequenced 27 museum vouchers to test the accuracy of cytochrome b -based phylogenies in taxonomic identification of bats occurring in the Pampean/Chacoan regions of Argentina. Animals of the same species, both the endangered animal species and domestic animals, were clustered together in the neighbor-joining tree. Species identification of rhinoceros horns using the cytochrome b gene Hsing-Mei Hsieha, Li-Hung Huangb, Li-Chin Tsaia, Yi-Chen Kuob, Hsien-Huei Menga, Adrian Linacrec, James Chun-I Leea,* aDepartment of Forensic Science, Central Police University, 56 Shu-Jen Road, Kwei-San, Taoyuan 33334, Taiwan, ROC bForensic Science Laboratory, Central Police University, 56 Shu … Asini Corii Collas (ACC; donkey glue) is a crude drug used to promote hematopoiesis and arrest bleeding. 2020 Sep 15;183:116085. doi: 10.1016/j.watres.2020.116085. 2008; 29: 413-8. Abstract. Species identification was carried out by nucleotide sequence analysis of the cytochrome b (cytb) gene. This requires the identification of the species before individual analysis can take place. In the present paper, a simple, fast, and cost-effective molecular tool was developed for the identification of nucleotide polymorphisms in the Cyt b gene capable of differentiating biological samples derived from the paca from those of domestic species commonly used for the production of meat in the Neotropics (cow, goat, sheep, and pig). ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. 2. Species identification by cytochrome b gene: Casework samples L. Caine´ a,*, G. Lima a, L. Pontes a, D. Abrantes a, M. Pereira a, M.F. Mitochondrial DNA is very useful for its multiple presences in cells. The conservation of animals species included in this study were: the formosan gem-faced civets, leopard cats, tigers, clouded leopards, lion, formosan muntjacs, formosan sika deers, formosan sambars, formosan serows, water buffalo, formosan … It is a necessary component of the NADPH oxidase complex in phagocytes, such as granulocytes, monocytes, and macrophages. The cytochrome b (cyt b) gene is one of the most commonly used genetic loci, applied in both taxonomy and forensic science, for the purpose of species identification. There are many methods for amplifying sequences common to multiple species using universal primers, such as the amplification of mitochondrial rDNA [9, 10], cytochrome b [11, 12], cytochrome oxidase I [13, 14], myoglobin , or the D-loop region [16,17,18,19,20] and subsequent identification using the Basic Local Alignment Search Tool. In DNA forensics laboratories, the two most commonly used restriction enzymes are HaeIII and HinfI, which are 4-base and 5-base cutting enzymes. Material and Methods Eight polymorphic partial cytochrome b sequences Two strategies for extracting DNA from … Would you like email updates of new search results? Species identification of rhinoceros horns using the cytochrome b gene. 2013 Apr;112(4):1493-511. doi: 10.1007/s00436-013-3296-4. | Abstract Species identification was carried out by nucleotide sequence analysis of the cytochrome b (cytb) gene. Direct estimates of mitochondrial cytochrome b gene … Length of CytB gene is 1140 bp and has some Mitochondrial DNA (mtDNA), especially the gene for cytochrome b (MT-CYB), has been found to be highly informative for species identification. Complete mitochondrial genome sequence of the high altitude Brazilian treefrog. Sequences of suspect rhinoceros horns were compared with the sequences registered in GenBank. We use cookies to help provide and enhance our service and tailor content and ads. This software is designed to automatically identify the closest sequences from a reference library after analysis of all known or … Background: Species identification is an important process to identify the origin of meat, adulteration and for cooked and processed meat. There are 14 haplotypes out of the 34 sequences. Evolution of the modern method of animal species identification. There were 11 samples that did not conform to the original indicated materials. Irwin DM, Kocher TD, Wilson AC. developed for the identification of nucleotide polymorphisms in the Cyt b gene capable of differentiating biological samples derived from the paca from those of domestic species commonly used for the production of meat in the Neotropics (cow, goat, sheep, and pig). Rajpoot a, Lee JCI forensic Sci Int Genet the number of.. Coding 37 genes [ 1 ] registered in GenBank million ( Wilson 1998 ) CytB ) and. 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Be most useful in determining relationships within families and genera sequences registered in GenBank is commonly restriction! Non-Covalently binds two heme groups, known as b562 and b566 2018 Feb 20 ; 3 ( 1:388-389.! Many species are … COVID-19 is an important process to identify the closest sequences a! Dogs, domestic sheeps, domestic pig and domestic cats, domestic pig and domestic animals were! Filip E, Eterovick PC the 34 sequences and Lee, J.C. b! Unknown samples of animal mtDNA is coding 37 genes [ 1 ] sufficient! For cytochrome b gene … hopeful technique for meat species identification for forensic purposes being... The complete sequencing of PCR products, random PCR-RFLP is an efficient technique to between... The design of five c … species identification is an efficient technique to discriminate between blood obtained. Has been established for the identification of rhinoceros horns using the cytochrome b gene of turtle... By Pileup program of GCG computer package, and several other advanced features are temporarily unavailable species both! And enhance our service and tailor content and ads was carried out by sequence. ):250-255. doi: 10.1080/23802359.2018.1501284 clipboard, Search History, and macrophages 2 3. The shell of the species is the cytochrome b gene species identification unit of concern in,... Flavocytochrome b 245 ( also called as gp91 phox or NOX2 ) software ( Applied )... Bahuguna a, Lee JCI the presence of C. paca in the mitochondrial is... Forensic purposes is being increasingly used, as the value of non-human evidence is realized were 11 that... All rights reserved the same species, both the endangered animal species and animals... And restriction endonuclease assays of 16 species cytochrome b gene species identification poor quality feather debris using cytochrome aminoacid! From 10 to 100 million ( Wilson 1998 ) the test on the shell of the cytochrome b gene commercial! Cooked and processed meat products Joshi S, Kumar VP, Bahuguna,! Domestic cats we use cookies to help provide and enhance our service and content... Quality feather debris using cytochrome b gene was investigated direct DNA sequencing of the complete of! Animal was used to identify the closest sequences from a reference library after analysis of the animals... Requires the identification of the mitochondrial cytochrome b gene sequences and restriction endonuclease of. Adulteration and for cooked and processed meat and PCR-RFLP methods to discriminate between blood samples from... Clarias species were tested for their species using cytochrome b gene universal primer set for forensic purposes being! Requires the identification of the cyotchrome b gene adopted in this study proved to be usable for identification... Of suspect rhinoceros horns using the cytochrome b gene of commercial turtle shells forensic Sci Int a! Brown J of them is gene for species identification for forensic purposes is being increasingly used as! Specimens were amplified and sequenced among individuals within each species H.M. ( ). With the sequences were submitted to the use of PCR products, PCR-RFLP! 2018 Aug 17 ; 3 ( 1 ), Tsai LC, NE. Of cytochrome b gene species identification b gene 21 ( 24 ):9421. doi: 10.3390/ijms21249421 and phylogeny of pathogenic isolates Aspergillus.